Bruno Maresca

Antonella Leone

Sergio Colonna-Romano

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Differential-Display Reverse Transcription-PCR (DDRT-PCR)

George S. Kobayashi

Hideyo Yamaguchi

Proceedings of the NATO Advanced Research Workshop on Molecular Biology and its Application to Medical Mycology, held at Taormina, Italy, January 6-8, 1992

Molecular Biology and its Application to Medical Mycology

Susan Lindquist

During the last years the heat shock response has been stu- died as a model system to analyze control mechanisms regula- ting the synthesis of heat shock proteins providing impor- tant general insight into the regulation of gene expression. But the major revelation, which has sparked interest from all quarters of biology, is the discovery that heat shock proteins play major roles in an extraordinary variety of normal cellular processes. They are the focus of investiga- tions in many areas of cell biology, including protein traf- ficking, signal transduction, DNS replication, transcrip- tion, protein synthesis, and in the assembly of di- verse protein structures. These aspects are thoroughly trea- ted in the book, as are the implications in immunology, in- fec- tious diseases, chronic degeneration, hyperthermia, and can- cer research.

Heat Shock

Bruno Tota

Guido di Prisco

Biology of Antarctic Fish presents the most recent findings on the biology of fish in the unique environment of the Antarctic ocean. At present the year-round temperature of the coastal waters is very near -1,87 ° C, the equilibrium temperature of the ice-seawater mixture. This extremely low temperature affects different levels of organization of fish life: individuals, organ systems, cells, organelles, membranes, and molecules. Exploring ecology, evolution, and life history as well as physiology, biochemistry, and molecular biology of Antarctic fish the book describes the mechanisms of cold adaptation at all these levels. It provides material for discussion also for fundamental questions in the field of adaptation to an extreme environment and therefore is of particular interest not only to specialized scientists, but also to those involved in basic and evolutionary biology.

Bruno Maresca

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