Christian Fleck

Rebecca E. McKenzie

Emma M Keizer

Jochem N.A Vink

Jasper van Lopik

Ferhat Büke

Vera Kalkman

Sander J Tans

Stan J.J Brouns

Abstract: While CRISPR-Cas defence mechanisms have been studied on a population level, their temporal dynamics and variability in individual cells have remained unknown. Using a microfluidic device, time-lapse microscopy and mathematical modelling, we studied invader clearance in Escherichia coli across multiple generations. We observed that CRISPR interference is fast with a narrow distribution of clearance times. In contrast, for invaders with escaping PAM mutations we found large cell-to-cell variability, which originates from primed CRISPR adaptation. Faster growth and cell division and higher levels of Cascade increase the chance of clearance by interference, while slower growth is associated with increased chances of clearance by priming. Our findings suggest that Cascade binding to the mutated invader DNA, rather than spacer integration, is the main source of priming heterogeneity. The highly stochastic nature of primed CRISPR adaptation implies that only subpopulations of bacteria are able to respond quickly to invading threats. We conjecture that CRISPR-Cas dynamics and heterogeneity at the cellular level are crucial to understanding the strategy of bacteria in their competition with other species and phages

Single Cell Variability of CRISPR-Cas Interference and Adaptation

How do intellectuals engage with and affect their publics? What is the role of the public intellectual in the new age of political uncertainties? What challenges face female intellectuals and those speaking from an ethnic, national or class position? This exciting collection responds to these questions by offering a broad-ranging account of the changing role of intellectuals in public life. The volume opens with provocative essays on the idea and role of the public intellectual from Alexander, Evans and Zulaika. Chapters from Rabinbach on intellectuals' responses to totalitarianism, Outhwaite on what it means to be a European intellectual, and Auer’s discussion of the dissident intellectual in the collapse of communism lead onto vigorous debate of earlier points discussed through specific intellectual case studies from Tocqueville to Hayek. Intellectuals and their Publics will attract a broad readership interested in the role of the intellectual, with particular appeal for sociologists, political theorists and historians of ideas.

Perspectives from the Social Sciences

Intellectuals and their Publics

Nico Stehr

Paul Neurath

During 1938 and 1939, Paul Neurath was a Jewish political prisoner in the concentration camps at Dachau and Buchenwald. He owed his survival to a temporary Nazi policy allowing release of prisoners who were willing to go into exile and the help of friends on the outside who helped him obtain a visa. He fled to Sweden before coming to the United States in 1941. In 1943, he completed The Society of Terror, based on his experiences in Dachau and Buchenwald. He embarked on a long career teaching sociology and statistics at universities in the United States and later in Vienna until his death in September 2001. After liberation, the horrific images of the extermination camps abounded from Dachau, Buchenwald, and other places. Neurath's chillingly factual discussion of his experience as an inmate and his astute observations of the conditions and the social structures in Dachau and Buchenwald captivate the reader, not only because of their authenticity, but also because of the work's proximity to the events and the absence of influence of later interpretations. His account is unique also because of the exceptional links Neurath establishes between personal experience and theoretical reflection, the persistent oscillation between the distanced and sober view of the scientist and that of the prisoner.

Inside the Dachau and Buchenwald Concentration Camps

Society of Terror

This book is available as open access through the Bloomsbury Open Access programme and is available on www.bloomsburycollections.com. From the beginning of the twentieth century, scientific and social scientific research has been characterised by intellectual exchange between Europe and the US. The establishment of the Third Reich ensured that, from the German speaking world, at least, this became a one-way traffic. In this book Christian Fleck explores the invention of empirical social research, which by 1950 had become the binding norm of international scholarship, and he analyses the contribution of German refugee social scientists to its establishment. The major names are here, from Adorno and Horkheimer to Hirshman and Lazarsfeld, but at the heart of the book is a unique collective biography based on original data from more than 800 German-speaking social scientists. Published in German in 2008 to great acclaim, Fleck's important study of the transatlantic enrichment of the social sciences is now available in a revised English-language edition.

Robber Barons, the Third Reich and the Invention of Empirical Social Research

A Transatlantic History of the Social Sciences

This ground-breaking volume is a follow-up to Intellectuals and Their Publics. In contrast to the earlier book, which was mainly concerned with the activity of intellectuals and how it relates to the public, this volume analyses what happens when sociology and sociologists engage with or serve various publics. More specifically, this problem will be studied from the following three angles: How does one become a public sociologist and prominent intellectual in the first place? (Part I) How complex and complicated are the stories of institutions and professional associations when they take on a public role or tackle a major social or political problem? (Part II) How can one investigate the relationship between individual sociologists and intellectuals and their various publics? (Part III) This book will be of interest to academics and students working in the fields of the sociology of knowledge and ideas, the history of social sciences, intellectual history, cultural sociology, and cultural studies.

Public Sociology in the Making

Knowledge for Whom?

Christian Fleck

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