John Maurer

This book will introduce non-molecular biologists to diagnostic PCR-based te- nologies for the detection of pathogens in foods. By the conclusion of this book, the reader should be able to: 1) understand the principles behind PCR including real-time; 2) know the basics involved in the design, optimization, and imp- mentation of PCR in food microbiology lab setting; 3) interpret results; 4) know limitations and strengths of PCR; and 5) understand the basic principles behind a new fledgling technology, microarrays and its potential applications in food microbiology. This book will provide readers with the latest information on PCR and microarray based tests and their application towards the detection of bacterial, protozoal and viral pathogens in foods. Figures, charts, and tables will be used, where appropriate, to help illustrate concepts or provide the reader with useful information or resources as an important starting point in bringing molecular diagnostics into the food microbiology lab. This book is not designed to be a “cookbook”PCR manual with recipes and step-by-step instructions but rather serve as a primer or resource book for students, faculty, and other professionals interested in molecular biology and its integration into food safety. v Table of Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Chapter 1. PCR Basics Amanda Fairchild, M. S. , Margie D. Lee DVM, Ph. D. , and John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. The Mythology of PCR: A Warning to the Wise John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Chapter 3.

PCR Methods in Foods

Erik Goldstein

The Washington Conference regulated the inter-war naval race between the world powers. In the era when it was still believed that battleships were the epitome of naval power and a sign of a country's strength, this conference led to limitations on the building of such weapons by the naval powers of Britain, the USA and Japan. This collection of essays deals with many aspects of the conference; the factors that caused it, the interests of the participating nations both present and future, and the results.

Naval Rivalry, East Asian Stability and the Road to Pearl Harbor

The Washington Conference, 1921-22

Christopher Bell

A great power arms race in naval weaponry and platforms, rising challengers seeking to overturn the existing international order in Asia, an economic slump that put immense pressure on politicians in democracies to trim defense budgets, and diplomatic efforts by statesmen to find ways to promote mutual security and avoid rivalries that could lead to war—all these features mark the current-day strategic environment. These features also marked in the era between the two world wars. To prevent the naval rivalries that augured international conflict, statesmen and naval leaders sought to negotiate arms control agreement. Their efforts to avert a great power naval arms race were crowned with achievement at the London Conference of 1930. What was accomplished at London, of course, did not prove lasting; nor did it lead to additional meaningful arms control and prevent future wars. Instead, London proved a dead end in the evolution of interwar international relations. The London Treaty marked the high point of interwar arms control. When measured against the magnitude of the international catastrophe that would unfold over the next decade, this achievement in arms control now appears practically meaningless at best and dangerous at worst. Critics of interwar arms control argue that, by weakening of American and British naval power, as well as stirring up extremist nationalism in Japanese internal politics, the London agreement represents a case study in political folly that contributed to the awful events leading to the war. The London Conference of 1930 thus represents a watershed, a turning point in the history of the interwar period. In this volume, leading naval historians tackle the question of how to assess the role played by naval arms control in the history of the interwar period. In addressing this important question, the authors uncover new evidence about the role of intelligence and behind-the-scenes political deal making that adds much to our knowledge of the international and naval history of this important era. This volume’s authors provide the first complete account of the strategic calculations and negotiations that shaped the outcome at the London Conference. No one interested in twentieth-century naval history, international relations and the rivalries of rising and declining great powers, and the origins of the Second World War can afford to miss this important new history.

The London Naval Conference in 1930

At the Crossroads Between Peace and War

Dr. Shawn Morgan finally meets the woman of his dreamsliterally, only to find out that Sarah Dooley is already engaged to someone else with a planned wedding in six months. Even though he wins a place in her heart, he steps aside so Sarah may move on with her life. Heartbroken, Shawn moves to Brazil to heal as well as to put lots of distance between them. Fate and developing fame become major reasons why he leaves Brazil for Dublin, Ireland where everything suddenly falls into place for him. After waiting on a woman for twelve years, Dr. Morgan decides to return to the United States where fate delivers him back to the woman of his dreams.

John Maurer

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