Piyush Pandey

M. Tofazzal Islam

Mahfuz Rahman

The third volume of the series Bacilli and Agrobiotechnology is comprised of 25 chapters that bring a unique perspective to the readers about Bacillus-mediated biotic and abiotic plant stress tolerance, bioremediation and bioprospecting. These chapters are prepared by the leading scientists of global repute. The negative impacts of agrochemicals such as chemical fertilizers and pesticides on human health and environment are paramount. Bacillus and allied genera of beneficial plant-associated microbes are presenting beacon of hope to the farmers, plant scientists and stewards of environment. Several chapters of this volume focus on the induction of various signaling pathways in plants by Bacillus spp. to alleviate biotic and abiotic stresses impacted by global climate change Agricultural lands contaminated with heavy metals affect the ecological food chain starting from crop cultivation. How the toxic effects of trace metals originating from industrial effluents and agrochemicals can be remediated? This book addresses how to overcome these issues by applying elite strains of Bacillus. Bioprospecting is a systematic and organized search for conversion of bioresources to industrially important products by utilizing microbe-derived metabolites. This volume is enriched by including the bioprospecting aspects mediated by Bacillus spp. with novel insights.

Plant Stress Tolerance, Bioremediation, and Bioprospecting

Bacilli in Agrobiotechnology

Kutubuddin Ali Molla

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Volume 2

CRISPR-Cas Methods

Chaitanya Kumar Jha

Abhinav Aeron

This volume of comprehensive reviews updates our knowledge of research and commercialization of Bacillus-based products in agriculture and the environmental sector. The last couple of decades have witnessed tremendous growth of research on Bacillus species. Many of these species can produce industrial enzymes, and can act simultaneously as biofertilizers and as biopesticides inhibiting important phytopathogens. This "biocontrol" activity is now elucidated by a number of genomic and metabolomic studies. Bacillus formulations are being patented and commercialized on a regular basis. Understanding the biology, ecology and mechanism of action of these bacteria will play a role in the promotion of Bacillus-based products to support green technology in agriculture and agro-based industries.

M. Tofazzal Islam

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