Shabir Hussain Wani

Mukesh Jain

Advances in molecular biology and genome research in the form of molecular breeding and genetic engineering put forward innovative prospects for improving productivity of many pulses crops. Pathways have been discovered, which include regulatory elements that modulate stress responses (e.g., transcription factors and protein kinases) and functional genes, which guard the cells (e.g., enzymes for generating protective metabolites and proteins). In addition, numerous quantitative trait loci (QTLs) associated with elevated stress tolerance have been cloned, resulting in the detection of critical genes for stress tolerance. Together these networks can be used to enhance stress tolerance in pulses. This book summarizes recent advances in pulse research for increasing productivity, improving biotic and abiotic stress tolerance, and enhancing nutritional quality.

Physiological, Molecular and Genetic Perspectives

Pulse Improvement

Rohini Garg

This volume looks at the latest techniques used by researchers to help them understand the biology of various cellular processes and agronomic traits, and come up with better strategies to improve legume crops. The chapters in this book cover topics such as legume genomic resources; legume pangenome and organelle genome construction; transcriptome analysis; DNA methylation analysis; double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture; genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures; genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and informative, Legume Genomics: Methods and Protocols is a useful reference for genomicists, molecular breeders, plant molecular biologists, biotechnologists, computational biologists, and developmental biologists. This book is also an excellent resource for any novice and expert researcher involved in various molecular aspects of legume biology or general plant studies.

Methods and Protocols

Legume Genomics

 Rohini Garg

Rajeev K Varshney

Abiotic stresses are the major cause that limits productivity of crop plants worldwide. Plants have developed intricate machinery to respond and adapt over these adverse environmental conditions both at physiological and molecular levels. Due to increasing problems of abiotic stresses, plant biotechnologists and breeders need to employ new approaches to improve abiotic stress tolerance in crop plants. Although current research has divulged several key genes, gene regulatory networks and quantitative trait loci that mediate plant responses to various abiotic stresses, the comprehensive understanding of this complex trait is still not available. This e-book is focused on molecular genetics and genomics approaches to understand the plant response/adaptation to various abiotic stresses. It includes different types of articles (original research, method, opinion and review) that provide current insights into different aspects of plant responses and adaptation to abiotic stresses.

Mukesh Jain

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