Patricia Marques

In an alternative present-day Lisbon, a small percentage of the population have been diagnosed as Gifted - having telepathic or telekinetic powers. Along with the power comes a stigma and suspicion that Gifted Inspector Isabel Reis with the Lisbon Police Force knows only too well. When Isabel is called to a investigate a mysterious and violent death which hints at Gifted involvement, she knows it's more than just her personal reputation on the line. In a society where Gifted individuals are already viewed with mistrust, Isabel is hiding her own secret and knows she has to tread carefully as a conspiracy emerges and the body count rises.

Rabbit Hole

'Breathtakingly original, and a captivating sense of place' Val McDermid, bestselling author of Still Life 'A brilliantly inventive and twisty tale' Claire McGowan, bestselling author of The Push 'An amazing genre-bending debut' David Jackson, bestselling author of The Resident The Murder In the Gare do Oriente, a body sits, slumped, in a stationary train. A high-profile man appears to have died by throwing himself repeatedly against the glass. But according to witnesses, he may not have done this of his own accord. The City Lisbon 2021. A small percentage of the population are diagnosed as Gifted. Along with the power comes stigma and suspicion. The Detective In a prejudiced city, Gifted Inspector Isabel Reis is hiding her own secrets while putting her life on the line to stop an ingenious killer. A violent and mysterious crime. Suspected Gifted involvement. A city baying for blood. And a killer who has only just begun . . . 'A brilliant read. The premise and characters are intriguing, the story completely compelling - I couldn't put it down - and I loved the beautifully depicted setting of Lisbon' Debbie Howells, bestselling author of The Bones of You 'This is crime fiction with a twist . . . This was a gripping and highly invented thrill ride. I can't wait for more' Nadine Matheson, author of The Jigsaw Man 'Patricia Marques crafts a riveting mystery about power, privilege, and murder. Genuinely gripping ' Lori M. Lee, author of Forest of Souls

A gripping crime novel set in the heart of Lisbon

The Colours of Death

Immunology and Serology are two major science fields. Immunology is defined as the study of the molecules, cells, organs, and systems responsible for the recognition and disposal of foreign material. Immunology began as a branch of microbiology. The study of infectious disease and the body's response to them has a major role for the development of immunology. Moreover, the concept of germ theory of disease has contributed to the field of immunology. It was Edward Jenner who first studied the response of the body to foreign substances. He observed that dairy maids who had naturally contracted a mild infection called cowpox seemed to be protected against smallpox, a horribly disfiguring disease and a major killer.Serology is the diagnostic identification of antibodies in the serum and other bodily fluids. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease).Serological tests may be performed for diagnostic purposes when an infection is suspected, in rheumatic illnesses, and in many other situations, such as checking an individual's blood type. Serology blood tests help to diagnose patients with certain immune deficiencies associated with the lack of antibodies, such as X-linked agammaglobulinemia. In such cases, tests for antibodies will be consistently negative.There are several serology techniques that can be used depending on the antibodies being studied. These include: ELISA, agglutination, precipitation, complement-fixation, and fluorescent antibodies and more recently chemiluminescence. Some serological tests are not limited to blood serum, but can also be performed on other bodily fluids such as semen and saliva, and Spinal fluid (CSF) which may contain antibodies.This book starts with a small historical introduction to Immunology. The next chapters (sections 1 to 4) give examples of Serology applied to infectious diseases (HPV, Hepatitis, Malaria and Dengue). Section 5 is dedicated to the application of serology to celiac diagnosis. Section 6 shows the application of serology to other pathogen (Lyme disease, Sjögren's syndrome, Chlamydia pneumoniae, HIV, Influenza virus, Mycobacterium, Toxoplasmosis and Leprosy). Several serologic based diagnostic techniques are used and are being developed daily, making this one of the biggest fields in science research.

Immunology and Serology

ELISA (enzyme-linked immunosorbent assay) revolutionized the way to detect antibodies and antigens, by using an enzymatic system instead of radioactive labelling. This techniques has been widely use since its development for disease diagnostics and other purposes. A quick Pubmed search will give you more than 23000 manuscripts peer-reviewed and published, using this assay. The ELISA technique was conceptualized and developed by Peter Perlmann, and Eva Engvall at Stockholm University, Sweden. Engvall and Perlmann published their first paper on ELISA in 1971. Solid-phase techniques were used in the development of microtiter plates (96 wells) in which either an antigen or an antibody is non-covalently bound to a solid-phase support. Technical advances led to automated pipetting devices (Micromedics; Hamilton), multichannel pipettes (Lab Systems), and microtiter plate readers and washers, and in the 1980s fully automated test instruments were manufactured by Boehringer-Mannheim and Abbott, among others. Such automated systems have come to stay in medical laboratories.This book starts by introducing the ELISA history and the three different main forms of the assay, Direct, Indirect, and Sandwich ELISA. It continues with a general overview of different ELISA methods using LPS, peptides as antigens and urine the sample for diagnosis (Section 1). The next chapters focus on different forms of the ELISA assays, peptide ELISA (Section 2), microarrays, microfluidics CD-ELISA and live-cell ELISA (Section 3). The next Sections are dedicated to the application of ELISA to the diagnosis of Malaria (Sections 4), other infectious diseases (Human papillomavirus, Dengue, Prions, Cystic Fibrosis, Leptospirosis, Herpes simplex related to HIV and Hepatitis C related to HIV (Section 5), different types of Cancer (Section 6) and Alzheimer's disease looking at biomarkers in sera and Cerebrospinal fluid (Section 7). This book is only a short overview of the big field that is the ELISA technique world. With new methods being developed daily. New methods combining this method with RFLP, PCR, microarray, microfluidics, live-cell imaging, and so on.New Infectious diseases, neurologic diseases, cancer and auto immune diseases diagnostic methods based on ELISA techniques are being commercialized and made available daily.

Theory and Practice

Elisa

The second in a speculative detective series set in Lisbon . . . Praise Patricia Marques' new series 'Breathtakingly original, and a captivating sense of place' Val McDermid, bestselling author of Still Life 'Compelling and original, this glints with freshness' Daily Mail 'A brilliantly inventive and twisty tale' Claire McGowan, bestselling author of The Push 'A good detective story . . . intriguing' Guardian 'A distinctive, intriguing, immersive debut' Mari Hannah, multi-award winning author of Without a Trace

Patricia Marques

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