C. Elegans Methods and Applications

The goal of C. elegans is to provide overviews and detailed step-by-step descriptions of newer and state-of-the-art methods utilized in the field. These include tools essential for forward and reverse genetic analysis, data mining, and comparative genomics strategies, electron and fluorescence microscopy methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and methods for physiological analyses including somatic cell culture, toxicity assays, electrophysiology, and in vivo imaging of intracellular Ca2+ and pH using genetically encoded fluorescent indicator proteins.