Cancer Cell Culture Methods and Protocols

This volume describes easy to follow methods to guide both the novice and more experienced researcher seeking to use new techniques for the culture of cancer cells. The first section of the book introduces the rationale behind the selection of specific materials to help the reader choose culture conditions appropriate to their studies and the general techniques operating in many culture facilities. The second section covers the specific requirements of the individual cancer cell types for optimal growth and maintenance. A wide range of procedures encompassing many of the key functional features of cancer cells are then described in section three. These include assays to evaluate proliferation, viability, cytotoxicity, apoptosis, migration, invasion, and angiogenesis. Techniques of gene transfer and the development of drug resistance are also described. Finally the book concludes with methods of co-culture of different cell types.